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Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers I, II, III of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

A validated UHPLC-MS method for tryptophan metabolites: Application in the diagnosis of multiple sclerosis

Ferenc Tömösi et. al
J. Pharm. Biomed. Anal., 185, 1-12 (2020)

Keywords: Tryptophan metabolism, Derivatization, Liquid chromatography-mass spectrometry, DryLab®4, Validation, Multiple sclerosis

PDF
http://doi.org/10.1016/j.jpba.2020.113246

Highlights:

 

  • Simultaneous and robust quantification of tryptophan and its 11 metabolites.
  • Derivatization; separation of analytes on pentafluorophenyl column.
  • Optimization of chromatographic separation using DryLab®4 software.
  • Quantification of analytes in human cerebrospinal fluid and serum.
  • Reference study for the clinical and scientific research of multiple sclerosis.

 

Rapid analysis of polycyclic aromatic hydrocarbons

Justin M. Godinho, Jason Lawhorn, Barry E. Boyes
J. Chromatogr. A, 1628, 27 September, 1-14 (2020)

Keywords: Polycyclic aromatic hydrocarbons, PAH, HPLC, Fast LC, Superficially porous particles

PDF
http://doi.org/10.1016/j.chroma.2020.461432

Highlights:

    • New superficially porous material for analyzing polycyclic aromatic hydrocarbons.
    • In silico modeling aided development of material use conditions.
    • Comparison between superficially porous and fully porous materials.
    • Good resolution for robust sub-minute separation of 16-component mix.

 

Development of a design of experiments optimized method for quantification of polysorbate 80 based on oleic acid using UHPLC-MS

J. Puschmann, D.-H. Evers, C. C. Müller-Goymann, M. E. Herbig
J. Chromatogr. A, 1599, 136-143 (2019)

Keywords: Polysorbate 80, Design of experiments, Quantification, Oleic acid, UHPLC-MS Peak separation, Cis/trans separation

PDF
http://doi.org/10.1016/j.chroma.2019.04.015

Highlights:

  • Alternative to gas chromatographic methods for fatty acid identification developed.
  • DoE optimized methods for peak separation of fatty acids and 18:1 isomers.
  • The DoE software Drylab was used to calculate optimized and robust methods for peak separation with a full factorial design.
  • Separation of cis/trans and structural fatty acid isomers using derivatization.
  • Indirect polysorbate 80 quantification (0.05–5.83 μg/mL) achieved using oleic acid.

 

Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 1: Alternative mobile phases and fine tuning of the separation

E. Farsang et. al
J Pharm Biomed Anal., 168, 10 May, 138-147 (2019)

Keywords: Cation-exchange chromatography, pH gradient, Salt gradient, Salt-mediated pH gradient, Monoclonal antibody, Method development

PDF
http://doi.org/10.1016/j.jpba.2019.02.024

Highlights:

  • Alternative buffer systems are suggested for CEX separation of mAbs.
  • Benefits of salt mediated pH gradient are discussed.
  • The combination of a “true” pH- and salt gradients extends the possibilities of method development.
  • Retention modeling and method optimization were performed by DryLab®4
  • Complete method development is feasible within 6 h.

 

Is hydrophobic interaction chromatography the most suitable technique to characterize site-specific antibody-drug conjugates?

V. D’Atri, R. Pell, A. Clarke, D. Guillarme, Sz. Fekete
J. Chromatogr. A, 1586, 8 February, 149-153 (2019)

Keywords: Site-specific antibody drug conjugates, Hydrophobic interaction chromatography, Reversed phase liquid chromatography, Mass spectrometry, Biopharmaceuticals

PDF
http://doi.org/10.1016/j.chroma.2018.12.020

Highlights:

  • ADC consists of a recombinant mAb covalently linked to a cytotoxic molecule.
  • Hydrophobic interaction chromatography represents the gold standard for ADC analysis.
  • New site-specific ADC formats are more homogeneous than their previous generations.
  • RPLC is proposed as alternative method to HIC for site-specific ADC characterization.
  • Retention and resolution modeling was performed with DryLab®4 software.

 

Computer-assisted UHPLC–MS method development and optimization for the determination of 24 antineoplastic drugs used in hospital pharmacy

N. Guichard, Sz. Fekete, D. Guillarme, P. Bonnabry, S. Fleury-Souverain
J Pharm Biomed Anal., 164, 5 February, 395-401 (2019)

Keywords: Retention modeling, Antineoplastic agent, Drylab, Method development, Reversed phase liquid chromatography

PDF
http://doi.org/10.1016/j.jpba.2018.11.014

This study reports the use of retention modeling software for the successful method development of 24 injectable antineoplastic agents. Firstly, a generic screening of several stationary and mobile phases (using various organic modifiers and pH) was achieved. Then, an optimization procedure of mobile phase temperature, gradient profile and mobile phase binary composition was conducted through only 28 real experiments using retention modeling software for data treatment. Finally, the optimized separation was achieved with a mobile phase consisting in 10 mM acetic acid at pH 5.1 (A) and acetonitrile (B). A Waters CORTECS® T3 column (100 × 2.1 mm, 1.6 μm) operated at 25 °C with a gradient time of 17.5 min (0-51%B) at a flow rate of 0.4 mL/min was used. The prediction offered by the retention model was found to be highly reliable, with an average error lower than 1%. A robustness testing step was also assessed from a virtual experimental design. Success rate and regression coefficient were evaluated without the need to perform any real experiment. The developed LC-MS method was successfully applied to the analysis of pharmaceutical formulations and wiping samples from working environment.

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