So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers IIIIII of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Computer-Assisted Approach for the Development of RP-HPLC Methods for the Separation and Quantification of Bioactive Plant Secondary Metabolites

Mohammed Séghir Daas, Massinissa Faci, Isabella Nicoletti, Malika Douzane, Danilo Corradini
Acta Pharm Hung, 91, 198-199 (2021)

Keywords: Plant secondary metabolites, RP-HPLC, design-of-experiments, Olea europaea, phenolic compounds



The optimization of RP-HPLC separations of plant secondary metabolites can be quickly and easily carried out using DryLab®, which predicts the chromatographic behavior of the analytes on the basis of a limited number of experiments. Such approach allows the development of robust and reliable RP-HPLC methods and reduces the consumption and waste of harmful and expensive organic solvents and, therefore, is both economic and ecologic.

A methodology employing retention modeling for achieving control space in liquid chromatography method development using quality by design approach

Karthik Jayaraman, Ashok Kumar Rajendran, Gandhi Santosh Kumar, Hemant Bhutani
J. Chromatogr. A, 1635, 4 January, 1-14 (2021)

Keywords: Liquid chromatography method development, Quality by Design, DryLab, Retention modeling, In-silico optimization, Robustness evaluation



  • Quality-by-design based liquid chromatographic method for new chemical entity
  • Exploration of design space using 2D and 3D chromatographic retention modeling
  • Establishment of a methodology for achieving analytical method control space
  • Demonstration of dependence of method control space on control strategy
  • Use of DryLab® to evaluate method robustness with acceptable accuracy


Negative gradient slope methods to improve the separation of closely eluting proteins

Szabolcs Fekete, Amarande Murisier, Jennifer M. Nguyen, Matthew A. Lauber, Davy Guillarme
J. Pharm. Biomed. Anal., 203, 5 September, 1-7 (2021)

Keywords: therapeutic proteins, monoclonal antibody, method development, gradient elution, negative gradient step, negative segmented multi-isocratic mode



In the present work, we describe the fundamental and practical advantages of a new strategy to improve the resolution of very closely eluting peaks within therapeutic protein samples.

This approach involves the use of multiple isocratic steps, together with the addition of a steep negative gradient segment (with a decrease in mobile phase strength) to "park" a slightly more retained peak somewhere along the column (at a given migration distance), while a slightly less retained compound can be eluted.

First, some model calculations were performed to highlight the potential of this innovative approach. For this purpose, the retention parameters (logk0 and S) for two case studies were considered, namely the analysis of a mixture of two therapeutic mAbs (simple to resolve sample) and separation of a therapeutic mAb from its main variant (challenging to resolve sample). The results confirm that the insertion of a negative segment into a multi-isocratic elution program can be a good tool to improve selectivity between critical peak pairs. However, it is also important to keep in mind that this approach only works with large solutes, which more or less follow an "on-off" type elution behavior.

Two real applications were successfully developed to illustrate the practical advantage of this new approach, including the separation of a therapeutic mAb from its main variant possessing very close elution behavior, and the separation of a carrier protein from an intact mAb as might be encountered in a quantitative bioanalysis assay. These two examples demonstrate that improved selectivity can be achieved for protein RPLC through the inclusion of a negative gradient slope that selectively bifurcates the elution of two or more peaks of interest.

Simultaneous determination of loading capacity and selectivity in preparative off-line two-dimensional separation: An application for purification of corilagin from Pomegranate flower extracts

Guliqire Adili et. al
Journal of Chromatography A, 1622, 1-9 (2020)

Keywords: Capacity orthogonal chromatography, Two-dimensional separation, Loading capacity, Orthogonality, Corilagin pomegranate flower extract



  • New method of 2D separation was developed with a use of HPLC-MS and DryLab software.
  • The method could optimize orthogonality and loading capacity simultaneously.
  • Theoretical fundamental and practical terms of the new method were discussed.
  • The method was used to purity 228 mg corilagin from pomegranate flower extracts.


High-performance liquid chromatography (HPLC)-fluorescence method for determination of bisphenol A diglycidyl ether (BADGE) and its derivatives in canned foods

Mingming Guo, Mingfeng He et al.
Science of The Total Environment, 710, 1-8 (2020)

Keywords: Bisphenol A diglycidyl ether (BADGE), HPLC, Core–shell particle column, Canned foods



  • HPLC-FLD method for BADGE and its derivatives analysis with LOD of 0.01–0.20 ng/g.
  • The optimization of separation conditions applying DryLab® software.
  • Adequate separation in 5 min using a core–shell particle column.



Improving selectivity and performing online on-column fractioning in liquid chromatography for the separation of therapeutic biopharmaceutical products

Sz. Fekete, H. Ritchie, J. Lawhorn, J.-L. Veuthey, D. Guillarme
Journal of Chromatography A, 1618, 1-9 (2020)

Keywords: Column coupling, Fractioning, Biopharmaceuticals, Mab, Improving selectivity



  • A novel column-coupling approach is suggested for large solute separations.
  • Widepore columns of different retentivity were selected for the coupling.
  • The suggested approach improves both the selectivity and efficiency.
  • The parameters of the linear solvent strength models were obtained using DryLab software. 
  • On-column protein fractioning can be rapidly performed.



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