So far, more than 230 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers IIIIII of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Software-supported HPLC Method Development for Separating Four Regioisomers of a Sugammadex-related Impurity

Dominika Herr, Arnold Zöldhegyi, Péter Soma Szakály, Erzsébet Varga
[Poster Presentation] HPLC 2023 Conference, Düsseldorf, Germany

Keywords: Di-OH-SGM, Qualification of Regioisomers, RP-HPLC modeling for Qualification, Critical method parameters, MS and NMR spectroscopy



  • A systematic modeling approach to contextualize data and optimize critical method parameters of regioisomers of Di-OH-SGM is provided
  • With this, the complex nature and chromatographic behavior of regioisomers of Di-OH-SGM is better understood
  • It is shown how to develop a simple and cost-effective RP-HPLC application for baseline-resolving regioisomers
  • Such an approach is required for the qualification of synthesized Di-OH-SGM material
  • The results of this work can be applied in various fields, including pharmaceuticals, biotechnology, and materials science


Analytical quality by design-compliant retention modeling for exploring column interchangeabilities in separating ezetimibe and its related substances

Elek Ferencz, Arnold Zöldhegyi, Éva-Katalin Kelemen, Mona Obreja, Imre Molnár
J. Chromatogr. A, 1682, 25 October, 1-11 (2022)

Keywords: Ezetimibe, QBD, DOE, Drylab, Column chemistry, Analytical Quality by Design (AQbD), Design Space Modeling



  • Computer-aided chromatographic method development for the separation of ezetimibe and its related substances
  • Design space comparison - new approach for testing column interchangeability
  • Mechanistic retention modeling and a 3D experimental design based approach via DryLab® software
  • Baseline separation of the analytes achieved on nine different stationary phases with various chemistries
  • Multivariate in silico robustness testing performed to identify the critical method parameters and to set up control strategy for routine applications
  • General specification with suitable working point yielding similar results on six stationary phases
  • Interchangeability of the columns proved in early phase method development


Expediting the chromatographic analysis of COVID-19 antibody therapeutics with ultra-short columns, retention modeling and automated method development

Bastiaan Duivelshof, Arnold Zöldhegyi, Davy Guillarme, Matthew Lauber, Szabolcs Fekete
J. Pharm. Biomed. Anal., 221, 30 November, 1-8 (2022)

Keywords: Covid-19, Casirivimab, Imdevimab, Ultra-short column, Retention modeling, Automation



  • Software assisted, automated approach was used for LC method development
  • Ultra-short protein columns enabled 1–2 min analysis time
  • Complete method development for four chromatographic modes required only 2 days
  • Conventional chromatographic techniques can quickly be transferred to short columns

Integrated analytical workflow for chromatographic profiling and metabolite annotation of a cytotoxic Phorbas amaranthus extract

Bruno S. do Amaral et. al
J. Chromatogr. B, 1174, 1 June, 1-10 (2021)

Keywords: Bioactivity, multi-column screening, chromatographic modeling and simulation, DryLab®, marine natural products



  • Phorbas amaranthus extract as natural products library
  • DryLab® software for Design of Experiments in association with scouting systems
  • Sixty-four metabolites chemically characterized by LC-HRMS
  • New metabolites inferred based on Global Natural Product Social Molecular Networking (GNPS)

Superheated reversed phase chromatography with ultrashort columns for the analysis of therapeutic proteins

Balázs Bobály, Alex Keresztfalvi, Thomas Gräber, Maria Anna Schwarz
J. Pharm. Biomed. Anal., 203, 5 September, 1-7 (2021)

Keywords: Superheated reversed phase chromatography, Non-covalent protein complexes, Ultrashort column, On-column degradation, Bispecific



  • Therapeutic protein complexes separated at superheated conditions.
  • Complex dissociation is complete at superheated conditions.
  • On-column degradation can be limited by fast gradients and ultrashort columns.

New wide-pore superficially porous stationary phases with low hydrophobicity applied for the analysis of monoclonal antibodies

Szabolcs Fekete et. al
J. Chromatogr. A, 1642, 12 April, 1-7 (2021)

Keywords: Superficially porous particles, wide-pore, low hydrophobicity, recovery, monoclonal antibody



  • New stationary phases with low hydrophobicity were tested for protein separations
  • The new ES-LH stationary phase showed excellent protein recovery
  • The ES-LH and C1 materials were found to be less retentive than commercial phases
  • The ES-LH phase showed novel selectivity due to its unique surface chemistry
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