Highlights

• Practical challenges of pH selection under reversed-phase conditions.   
• Using Design Space modeling to capture dynamic separation behavior of volatile and non-volatile buffer systems.
• Applying Design Space Comparison for in-depth buffer interchangeability studies.  
• Research outlook: Selectivity differences observed with NH₄⁺, K⁺, Na⁺, and Li⁺ buffer cations, highlighting varying salting-out effects.

Highlights 

• Allopurinol and its Ph.Eur. impurities are analyzed with porous graphitic carbon.
• The retention behavior is different than in reversed phase separations.
• Linear solvent strength (LSS) theory can be used for method development.
• All compounds can be separated in 6 min with baseline resolution.
• LSS-based method development software are efficient for PGC phases.

Highlights 

• Computer-Assisted multifactorial chromatographic strategies were addressed.
• Using proper linear and non-linear models were discussed.
• Applications in various chromatographic techniques are critically reviewed.
• Important parameters for retention mechanism modelling are demonstrated.
• Computer-Assisted optimization strategies are highlighted for generic methods.

Highlights 

• Vitamin A isomer separation in RP mode on CSPs is superior to existing methods.
• Screening of CSPs provides ideal stationary phase.
• Molecular planarity recognition is the predominant retention mechanism.
• Method optimization and considerations for a better suited stationary phase design.

Highlights 

• Introducing modeling methodology for a robust assessment of UHPLC column provided selectivities.
• Multidimensional Design Spaces offering AQbD-compliant alternative for column selectivity testing and comparison methods.
• Identifying shared method conditions with established interchangeability between various C18 stationary phases.
• Pharmaceutical application: optimal workpoints, substitute columns, ensure consistency, identification of equivalent/orthogonal column selectivities.