So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers IIIIII of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Improving selectivity and performing online on-column fractioning in liquid chromatography for the separation of therapeutic biopharmaceutical products

Sz. Fekete, H. Ritchie, J. Lawhorn, J.-L. Veuthey, D. Guillarme
Journal of Chromatography A, 1618, 1-9 (2020)

Keywords: Column coupling, Fractioning, Biopharmaceuticals, Mab, Improving selectivity



  • A novel column-coupling approach is suggested for large solute separations.
  • Widepore columns of different retentivity were selected for the coupling.
  • The suggested approach improves both the selectivity and efficiency.
  • The parameters of the linear solvent strength models were obtained using DryLab software. 
  • On-column protein fractioning can be rapidly performed.



Development of a generic reversed-phase liquid chromatography method for protein quantification using analytical quality-by-design principles

Julian Kopp et al.
Journal of Pharmaceutical and Biomedical Analysis, 188, 1-9 (2020)

Keywords: Liquid chromatography, (U)HPLC, Protein quantification, E. coli, Analytical quality by design (AQbD), Design of Experiment (DoE)



  • DryLab Assisted modeling of secondary method factors.
  • Timely and accurate analytics needed for recombinant protein production.
  • Chromatographic modeling accelerated method development using an AQbD approach.
  • Robustness of Method Operable Design Region tested in-silico and experimentally.
  • Developed RPLC-Method is superior to current analytical techniques.


Updating the European Pharmacopoeia impurity profiling method for terazosin and suggesting alternative columns

D. Enesei, I. Kapui, Sz. Fekete, R. Kormány
J. Pharm. Biomed. Anal., 187, 1-10 (2020)

Keywords: Column comparison, Design of experiments, Method validation, Quality by design, Robustness, Terazosin



  • A new (U)HPLC method was developed for terazosin impurity profiling with DryLab to update the Ph. Eur. Method.
  • A generic workflow has been proposed to suggest replacement columns for a given separation.
  • Column comparison was based on the visualization and overlay of design spaces.
  • The new method was validated in accordance with international guidelines.


Structure-Function Assessment and High-Throughput Quantification of Site-Specific Aspartate Isomerization in Monoclonal Antibody Using a Novel Analytical Tool Kit

Kaimeng Zhou et al.
Journal of Pharmaceutical Sciences, 109, 1, 422-428 (2020)

Keywords: antibody, proteins, formulation, post-translational modification, isomerization, succinimide, liquid chromatography, peptide mapping, stability, structure-activity relationship


Isomerization of surface-exposed aspartic acid (Asp) in the complementarity-determining regions of therapeutic proteins could potentially impact their target binding affinity because of the sensitive location, and often requires complex analytical tactics to understand its effect on structure-function and stability. Inaccurate quantitation of Asp-isomerized variants, especially the succinimide intermediate, presents major challenge in understanding Asp degradation kinetics, its stability, and consequently establishing a robust control strategy. As a practical solution to this problem, a comprehensive analytical tool kit has been developed, which provides a solution to fully characterize and accurately quantify the Asp-related product variants. The toolkit offers a combination of 2 steps, an ion-exchange chromatography method to separate and enrich the isomerized variants in the folded structure for structure-function evaluation and a novel focused peptide mapping method to quantify the individual complementarity-determining region isomerization components including the unmodified Asp, succinimide, and isoaspartate. This novel procedure allowed an accurate quantification of each Asp-related variant and a comprehensive assessment of the functional impact of Asp isomerization, which ultimately helped to establish an appropriate control strategy for this critical quality attribute.

A validated UHPLC-MS method for tryptophan metabolites: Application in the diagnosis of multiple sclerosis

Ferenc Tömösi et. al
J. Pharm. Biomed. Anal., 185, 1-12 (2020)

Keywords: Tryptophan metabolism, Derivatization, Liquid chromatography-mass spectrometry, DryLab®4, Validation, Multiple sclerosis




  • Simultaneous and robust quantification of tryptophan and its 11 metabolites.
  • Derivatization; separation of analytes on pentafluorophenyl column.
  • Optimization of chromatographic separation using DryLab®4 software.
  • Quantification of analytes in human cerebrospinal fluid and serum.
  • Reference study for the clinical and scientific research of multiple sclerosis.


Rapid analysis of polycyclic aromatic hydrocarbons

Justin M. Godinho, Jason Lawhorn, Barry E. Boyes
J. Chromatogr. A, 1628, 27 September, 1-14 (2020)

Keywords: Polycyclic aromatic hydrocarbons, PAH, HPLC, Fast LC, Superficially porous particles



    • New superficially porous material for analyzing polycyclic aromatic hydrocarbons.
    • In silico modeling aided development of material use conditions.
    • Comparison between superficially porous and fully porous materials.
    • Good resolution for robust sub-minute separation of 16-component mix.


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