Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers IIIIII of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Analytical quality by design-compliant retention modeling for exploring column interchangeabilities in separating ezetimibe and its related substances

Elek Ferencz, Arnold Zöldhegyi, Éva-Katalin Kelemen, Mona Obreja, Imre Molnár
J. Chromatogr. A, 1682, 25 October, 1-11 (2022)

Keywords: Ezetimibe, QBD, DOE, Drylab, Column chemistry, Analytical Quality by Design (AQbD), Design Space Modeling

PDF
http://doi.org/10.1016/j.chroma.2022.463494

Highlights

  • Computer-aided chromatographic method development for the separation of ezetimibe and its related substances
  • Design space comparison - new approach for testing column interchangeability
  • Mechanistic retention modeling and a 3D experimental design based approach via DryLab® software
  • Baseline separation of the analytes achieved on nine different stationary phases with various chemistries
  • Multivariate in silico robustness testing performed to identify the critical method parameters and to set up control strategy for routine applications
  • General specification with suitable working point yielding similar results on six stationary phases
  • Interchangeability of the columns proved in early phase method development

 


Serum bile acids in cystic fibrosis patients – glycodeoxycholic acid as a potential marker of liver disease

Drzymała-Czyz et al.
Digestive and Liver Disease, 54, January, 111-117 (2022)

Keywords: Cholic acid, Cystic fibrosis liver disease, Deoxycholic acid, Liver cirrhosis

PDF
http://doi.org/10.1016/j.dld.2021.06.034

Abstract

Background: Cystic fibrosis (CF) and CF-related liver disease can lead to disturbances in bile acid metabolism.
Aim: This study determined serum bile acid concentrations in CF to define their usefulness in liver disease assessment.
Methods: Primary, secondary and conjugated bile acid levels were measured in three CF groups (25 patients each) exhibiting: liver cirrhosis, other liver disease, no liver disease, and in 25 healthy subjects (HS).
Results: Bile acid levels were higher in CF patients than in HS, except for glycodeoxycholic acid (GDCA). However, bile acid concentrations did not differ between patients with cirrhosis and other liver involvement. GDCA and deoxycholic acid (DCA) differentiated CF patients with non-cirrhotic liver disease from those without liver disease (GDCA-AUC: 0.924, 95%CI 0.822–1.000, p<0.001; DCA-AUC: 0.867, 95%CI: 0.731–1.000, p<0.001). Principal component analysis revealed that in CF liver disease was related to GDCA, GGTP activity, severe genotype and pancreatic insufficiency.
Conclusions: A CF-specific bile acid profile was defined and shown to relate to liver disease. GDCA differentiates patients with non-cirrhotic liver involvement from those with no detectable liver disease. Hence, GDCA is a candidate for validation as a biomarker of non-cirrhotic progression of liver disease in CF.


Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals

Simon Jaag, Chunmei Wen, Benjamin Peters, Michael Lämmerhofer
Journal of Chromatography A, 1676, 2 August, 1-13 (2022)

Keywords: Gradient kinetic plots, Monolithic columns, Core shell particle, Fully porous particle, UHPLC

PDF
http://doi.org/10.1016/j.chroma.2022.463251

Highlights

  • Kinetic performance comparison of protein reversed-phase columns.
  • Model proteins of various size, NISTmAb and NISTmAb fragments as test analytes.
  • Core-shell, fully porous & monolithic columns compared using gradient kinetic plots.
  • Kinetic plots allow convenient comparison in terms of speed or max. plate numbers.
  • 400/450 Å core-shell columns showed the best performance due to a thin shell.

 


Computer-assisted UHPLC method development and optimization for the determination of albendazole and its related substances

Elek Ferencz et. al
J. Pharm. Biomed. Anal., 203, 5 September, 1-11 (2021)

Keywords: Drylab, Retention modelling, AQbD, Experimental design, Method validation, In silico robustness testing

PDF
http://doi.org/10.1016/j.jpba.2021.114203

Highlights

• Computer aided chromatographic method development for the simultaneous determination of albendazole and its related substances.

• Optimization was realized using experimental design-based approach.

• Alternative to replace an underperforming European Pharmacopoeia method.

• In silico robustness testing and retention modelling performed by DryLab software.

• After validation, method applicability was tested on commercial tablet formulation.


Superheated reversed phase chromatography with ultrashort columns for the analysis of therapeutic proteins

Balázs Bobály, Alex Keresztfalvi, Thomas Gräber, Maria Anna Schwarz
J. Pharm. Biomed. Anal., 203, 5 September, 1-7 (2021)

Keywords: Superheated reversed phase chromatography, Non-covalent protein complexes, Ultrashort column, On-column degradation, Bispecific

PDF
http://doi.org/10.1016/j.jpba.2021.114162

Highlights

  • Therapeutic protein complexes separated at superheated conditions.
  • Complex dissociation is complete at superheated conditions.
  • On-column degradation can be limited by fast gradients and ultrashort columns.

New wide-pore superficially porous stationary phases with low hydrophobicity applied for the analysis of monoclonal antibodies

Szabolcs Fekete et. al
J. Chromatogr. A, 1642, 12 April, 1-7 (2021)

Keywords: Superficially porous particles, wide-pore, low hydrophobicity, recovery, monoclonal antibody

PDF
http://doi.org/10.1016/j.chroma.2021.462050

Highlights

  • New stationary phases with low hydrophobicity were tested for protein separations
  • The new ES-LH stationary phase showed excellent protein recovery
  • The ES-LH and C1 materials were found to be less retentive than commercial phases
  • The ES-LH phase showed novel selectivity due to its unique surface chemistry
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