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Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers I, II, III of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Ion-pair reversed-phase chromatography analysis of oligonucleotides using ultra-short (20 x 2.1 mm) columns. Tutorial

Szabolcs Fekete, Mateusz Imiolek, Matthew Lauber
Journal of Chromatography Open, 6, November, 1-9 (2024)

Keywords: Oligonucleotide separation, Ion-pairing, Retention modeling, Ultra-short column, Platform method

PDF
http://doi.org/10.1016/j.jcoa.2024.100187

Highlights 

  • Comprehensive guide to the analysis of oligonucleotides using ultra-short columns.
  • The effect of ion-pairing agent on selectivity is explained.
  • Software-assisted retention modeling allows very fast (1 day) method optimization.
  • Concave gradients can be considered as platform (generic) methods.

Software-supported HPLC Method Development for Separating Four Regioisomers of a Sugammadex-related Impurity

Dominika Herr, Arnold Zöldhegyi, Péter Soma Szakály, Erzsébet Varga
[Poster Presentation] HPLC 2023 Conference, Düsseldorf, Germany

Keywords: Di-OH-SGM, Qualification of Regioisomers, RP-HPLC modeling for Qualification, Critical method parameters, MS and NMR spectroscopy

PDF

Highlights

  • A systematic modeling approach to contextualize data and optimize critical method parameters of regioisomers of Di-OH-SGM is provided
  • With this, the complex nature and chromatographic behavior of regioisomers of Di-OH-SGM is better understood
  • It is shown how to develop a simple and cost-effective RP-HPLC application for baseline-resolving regioisomers
  • Such an approach is required for the qualification of synthesized Di-OH-SGM material
  • The results of this work can be applied in various fields, including pharmaceuticals, biotechnology, and materials science

 

Expediting the chromatographic analysis of COVID-19 antibody therapeutics with ultra-short columns, retention modeling and automated method development

Bastiaan Duivelshof, Arnold Zöldhegyi, Davy Guillarme, Matthew Lauber, Szabolcs Fekete
J. Pharm. Biomed. Anal., 221, 30 November, 1-8 (2022)

Keywords: Covid-19, Casirivimab, Imdevimab, Ultra-short column, Retention modeling, Automation

PDF
http://doi.org/10.1016/j.jpba.2022.115039

Highlights:

  • Software assisted, automated approach was used for LC method development
  • Ultra-short protein columns enabled 1–2 min analysis time
  • Complete method development for four chromatographic modes required only 2 days
  • Conventional chromatographic techniques can quickly be transferred to short columns

Analytical quality by design-compliant retention modeling for exploring column interchangeabilities in separating ezetimibe and its related substances

Elek Ferencz, Arnold Zöldhegyi, Éva-Katalin Kelemen, Mona Obreja, Imre Molnár
J. Chromatogr. A, 1682, 25 October, 1-11 (2022)

Keywords: Ezetimibe, QBD, DOE, Drylab, Column chemistry, Analytical Quality by Design (AQbD), Design Space Modeling

PDF
http://doi.org/10.1016/j.chroma.2022.463494

Highlights

  • Computer-aided chromatographic method development for the separation of ezetimibe and its related substances
  • Design space comparison - new approach for testing column interchangeability
  • Mechanistic retention modeling and a 3D experimental design based approach via DryLab® software
  • Baseline separation of the analytes achieved on nine different stationary phases with various chemistries
  • Multivariate in silico robustness testing performed to identify the critical method parameters and to set up control strategy for routine applications
  • General specification with suitable working point yielding similar results on six stationary phases
  • Interchangeability of the columns proved in early phase method development

 

Computer-assisted UHPLC method development and optimization for the determination of albendazole and its related substances

Elek Ferencz et. al
J. Pharm. Biomed. Anal., 203, 5 September, 1-11 (2021)

Keywords: Drylab, Retention modelling, AQbD, Experimental design, Method validation, In silico robustness testing

PDF
http://doi.org/10.1016/j.jpba.2021.114203

Highlights

• Computer aided chromatographic method development for the simultaneous determination of albendazole and its related substances.

• Optimization was realized using experimental design-based approach.

• Alternative to replace an underperforming European Pharmacopoeia method.

• In silico robustness testing and retention modelling performed by DryLab software.

• After validation, method applicability was tested on commercial tablet formulation.

Superheated reversed phase chromatography with ultrashort columns for the analysis of therapeutic proteins

Balázs Bobály, Alex Keresztfalvi, Thomas Gräber, Maria Anna Schwarz
J. Pharm. Biomed. Anal., 203, 5 September, 1-7 (2021)

Keywords: Superheated reversed phase chromatography, Non-covalent protein complexes, Ultrashort column, On-column degradation, Bispecific

PDF
http://doi.org/10.1016/j.jpba.2021.114162

Highlights

  • Therapeutic protein complexes separated at superheated conditions.
  • Complex dissociation is complete at superheated conditions.
  • On-column degradation can be limited by fast gradients and ultrashort columns.
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