Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers IIIIII of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Revisiting column selectivity choices in ultra-high performance liquid chromatography–Using multidimensional analytical Design Spaces to identify column equivalency

Arnold Zöldhegyi, Krisztián Horváth, Róbert Kormány
Journal of Chromatography A, 1719, 29 March, 1-10 (2024)

Keywords: Analytical quality by design, Method operable design region, Replacement columns, Design space modeling, Qualifying similarity/orthogonality in HPLC

PDF
http://doi.org/10.1016/j.chroma.2024.464738

Highlights 

  • Introducing modeling methodology for a robust assessment of UHPLC column provided selectivities.
  • Multidimensional Design Spaces offering AQbD-compliant alternative for column selectivity testing and comparison methods.
  • Identifying shared method conditions with established interchangeability between various C18 stationary phases.
  • Pharmaceutical application: optimal workpoints, substitute columns, ensure consistency, identification of equivalent/orthogonal column selectivities.  

 


Retention modeling of therapeutic peptides in sub-/supercritical fluid chromatography

Jonas Neumann, Sebastian Schmidtsdorff, Alexander H. Schmidt, Maria K. Parr
Separation Science Plus, 7, 11 February, 1-12 (2024)

Keywords: Chromatographic modeling, Retention time prediction, Ternary composition, Therapeutic peptides

PDF
http://doi.org/10.1002/sscp.202300239

Abstract

Chromatographic modeling software packages are valuable tools during method optimization steps. These are well established for reversed‐phase applications utilizing retention time (RT) prediction to optimize separations and receive robust methods, which is of high interest for the analysis of pharmaceuticals. In contrast to liquid chromatography, the knowledge of RT prediction in supercritical fluid chromatography is limited to a manageable number of studies.

This study uses the software DryLab to predict the RTs of the peptides bacitracin (Bac), colistin, tyrothricin (Tyro), and insulin analogs. Gradient time, column temperature, and the ternary composition (terC) of carbon dioxide, methanol (MeOH), and acetonitrile (ACN) in the gradient elution are varied in a feasibility approach using a neutral (Viridis BEH) and an amino‐derivatized aromatic (Torus 2‐PIC) stationary phase. In the second part, chromatographic optimization is performed in silico through gradient adjustments to optimize the separation of the fingerprint of Bac. The final gradient method utilizes a Viridis BEH column (100 × 3.0 mm, 1.7 μm), carbon dioxide, and a modifier consisting of ACN/MeOH/water/methanesulfonic acid (60:40:2:0.1, v:v:v:v). In addition, changes in the retention order of Tyro compounds with the proportion of the terC in combination with a Torus Diol column are investigated.

 

 


Ion-pair reversed-phase chromatography analysis of oligonucleotides using ultra-short (20 x 2.1 mm) columns. Tutorial

Szabolcs Fekete, Mateusz Imiolek, Matthew Lauber
Journal of Chromatography Open, 6, November, 1-9 (2024)

Keywords: Oligonucleotide separation, Ion-pairing, Retention modeling, Ultra-short column, Platform method

PDF
http://doi.org/10.1016/j.jcoa.2024.100187

Highlights 

  • Comprehensive guide to the analysis of oligonucleotides using ultra-short columns.
  • The effect of ion-pairing agent on selectivity is explained.
  • Software-assisted retention modeling allows very fast (1 day) method optimization.
  • Concave gradients can be considered as platform (generic) methods.

Updating the European Pharmacopoeia impurity profiling method for cetirizine and suggesting alternative column, using Design Space Comparison

Róbert Kormány, Barnabás Soós, Krisztián Horváth
Journal of Pharmaceutical and Biomedical Analysis, 237, 5 January , 1-9 (2024)

Keywords: Column comparison, Design of experiments, Method validation, Quality by design, Robustness, Cetirizine

PDF
http://doi.org/10.1016/j.jpba.2023.115776

Highlights

  • A new UHPLC method was developed for cetirizine impurity profiling to update the Ph. Eur. method.
  • A generic workflow has been proposed to suggest replacement columns for a given separation.
  • Column comparison was based on the visualization and overlay of design spaces.
  • The new method was validated in accordance with international guidelines.

 


Software-supported HPLC Method Development for Separating Four Regioisomers of a Sugammadex-related Impurity

Dominika Herr, Arnold Zöldhegyi, Péter Soma Szakály, Erzsébet Varga
[Poster Presentation] HPLC 2023 Conference, Düsseldorf, Germany

Keywords: Di-OH-SGM, Qualification of Regioisomers, RP-HPLC modeling for Qualification, Critical method parameters, MS and NMR spectroscopy

PDF

Highlights

  • A systematic modeling approach to contextualize data and optimize critical method parameters of regioisomers of Di-OH-SGM is provided
  • With this, the complex nature and chromatographic behavior of regioisomers of Di-OH-SGM is better understood
  • It is shown how to develop a simple and cost-effective RP-HPLC application for baseline-resolving regioisomers
  • Such an approach is required for the qualification of synthesized Di-OH-SGM material
  • The results of this work can be applied in various fields, including pharmaceuticals, biotechnology, and materials science

 


Expediting the chromatographic analysis of COVID-19 antibody therapeutics with ultra-short columns, retention modeling and automated method development

Bastiaan Duivelshof, Arnold Zöldhegyi, Davy Guillarme, Matthew Lauber, Szabolcs Fekete
J. Pharm. Biomed. Anal., 221, 30 November, 1-8 (2022)

Keywords: Covid-19, Casirivimab, Imdevimab, Ultra-short column, Retention modeling, Automation

PDF
http://doi.org/10.1016/j.jpba.2022.115039

Highlights:

  • Software assisted, automated approach was used for LC method development
  • Ultra-short protein columns enabled 1–2 min analysis time
  • Complete method development for four chromatographic modes required only 2 days
  • Conventional chromatographic techniques can quickly be transferred to short columns
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