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Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers I, II, III of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Keyword Year

Overcoming challenges in LC separation of oxysterols through guided design space modelling: A comparison of three stationary phases

Andrea Castellaneta et. al
Journal of Chromatography A, 1770, 15 March, 1-11 (2026)

Keywords: Oxysterols, DryLab®, Analytical design space modelling, Reversed-phase liquid chromatography, High-resolution mass spectrometry

http://doi.org/10.1016/j.chroma.2026.466735

Highlights

  • Development of HPLC methods resolved ambiguities in MS-based detection of oxysterols.
  • Design space modeling was applied to optimize HPLC separation of native oxysterols.
  • The best performance was obtained using cyanopropyl and pentafluorophenyl columns.
  • Insights on in-source fragmentation dynamics could differentiate isomeric oxysterols.

Extended multidimensional design space studies: Comparing volatile and non-volatile buffer systems in UPLC

Arnold Zöldhegyi, Barnabás Soós, Krisztián Horváth, Imre Molnár, Róbert Kormány
Journal of Chromatography A, 1751, 21 June, 1-11 (2025)

Keywords: pH-selection in reversed phase chromatography, Volatile Non-volatile buffer interchangeability, Salting-out Effects, Analytical quality by design, Design space comparison

http://doi.org/10.1016/j.chroma.2025.465951

Highlights

  • Practical challenges of pH selection under reversed-phase conditions.   
  • Using Design Space modeling to capture dynamic separation behavior of volatile and non-volatile buffer systems.
  • Applying Design Space Comparison for in-depth buffer interchangeability studies.  
  • Research outlook: Selectivity differences observed with NH₄⁺, K⁺, Na⁺, and Li⁺ buffer cations, highlighting varying salting-out effects.

 

Weak to strong ion-pair gradients to expand the selectivity of oligonucleotide separations in reversed phase liquid chromatography - A proof of concept

Szabolcs Fekete, Mateusz Imiolek, Matthew Lauber
Journal of Chromatography Open, 7, May, 1-8 (2025)

Keywords: Ion-pair reversed phase chromatography, Oligonucleotide separation, Dual-gradient, Selectivity, Ultra-short column

PDF
http://doi.org/10.1016/j.jcoa.2024.100200

Highlights

  • A dual gradient approach is proposed to enhance the separation of oligonucleotides.
  • Running a weak to strong ion pair gradient significantly expands the elution window.
  • Combination of dual gradient with concave gradient program shows further benefits.
  • The degree of freedom can be significantly improved to extend the design space.

 

A novel 3D-printed sample preparation method for benzodiazepine quantification in human serum

Dagmara Kroll et. al
Analytica Chimica Acta, 1337, 1 February, 1-8 (2025)

Keywords: Benzodiazepines, 3D printing, Solid-phase microextraction, Sample preparation, Fused deposition modelling, Human serum

PDF
http://doi.org/10.1016/j.aca.2024.343552

Highlights

  • Extraction utilizing 3D-printed sorbent devices.
  • Devices made with a thermoplastic material with incorporated C18 silica.
  • Sample preparation fully validated in human serum according to ICH guideline.
  • A DryLab optimized LC-MS method for separation of 11 benzodiazepines.
  • A protocol suitable for toxicological and medical analyses of real samples.

 

Leveraging Multidimensional Modeling to Resolve Frequent Separation Challenges in HPLC

Arnold Zoldhegyi, Róbert Kormány, Szabolcs Fekete, Krisztián Horváth, Imre Molnár
Column, 21, 3, 14-20 (2025)

http://www.chromatographyonline.com/view/levera...

Highlights

  • Gas chromatography and HPLC modeling have evolved, providing diverse tools for method development and chromatographic behavior prediction.
  • Empirical DoE and first-principles approaches offer distinct advantages in method development, with multidimensional models enhancing pharmaceutical research.
  • Design space comparison tools address challenges like column interchangeability, batch-to-batch reproducibility, and system comparisons, supporting robust HPLC analysis.
  • Recent studies highlight the importance of systematic modeling approaches in addressing practical challenges in routine analytical work.

Case Study: Robustness Investigations

Gerd Kleinschmidt, Birgit Niederhaus
Method Validation in Pharmaceutical Analysis, Joachim Ermer, Phil Nethercote, (Wiley‐VCH GmbH, 3, 2025), 301-341 / Chapter 11

http://doi.org/10.1002/9783527831708.ch11
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