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Frequently Asked Questions

1. What kind of sample compounds is DryLab good for?

DryLab can work with all types of compounds, including small and large molecules.  

2. Which chromatographic data systems does DryLab work with?

DryLab works with all chromatographic data systems. It reads the universal import/export format AIA (Analytical Instrument Association), which is available for “Export” as *.cdf files from any integration software.  

3. How accurate is DryLab?

DryLab predicts retention times with over 99% accuracy. We have ca. 180 publications documenting DryLab’s amazing predictive power! 

4. What input-experiment conditions do you recommend for building a 3D model?

When building a 3D model you can choose either the tG-T-pH mode or the tG-T-tC mode.  

  • tG: time of gradient
  • T: temperature
  • tC: ternary composition of eluent B

We recommend the following conditions:

Gradient Time (tG): choose two run times, differing by a factor of three.  For example, for a column of 50 x 2.1mm, 1.7µm, you can use gradient times of 3 min and 9 min, with a gradient from 5 to 95 %B.  

Temperature (T): choose two values, differing by 30°C. For example, 30°C and 60°C, or 40°C and 70°C.

pH: choose 3 values, differing by 0.6 between each consecutive value. For example, 2.0; 2.6; 3.2

Ternary Composition %B2 in B1 (tC): choose three values, for example, one with 100% acetonitrile, one with a mixture of acetonitrile and methanol (50:50)(V:V), and one with 100% methanol.

Please note that ALL other conditions must be constant across all input runs. 

5. How do I import my method data into DryLab?

You have two options for importing your input runs.

A. Upload *.cdf files

Once you’re run your input experiments, you should integrate your chromatograms to include retention times and peak areas, and export them from your integration software in AIA format (as *.cdf files). Then you can simply upload them one-by-one on the data entry page.

B. Copy/Paste your peak table:

Alternatively, you can copy and paste your peak tables (with peak areas and retention times) from Excel into the peak-tracking page of data entry.

6. I have coeluting peaks in my input runs. Will this be a problem?

No! Co-eluting peaks during input runs are expected, and DryLab’s peak tracking tool has a number of useful features for organizing your peak table, including an automatic peak-splitting button.  

7. My separation almost always has tailing peaks. Can DryLab model this?

Yes! You have the option of entering peak-tailing data during the peak tracking process. DryLab will use this data to give you a realistic prediction of what your separation will look like when you run your DryLab conditions in the laboratory. 

8. My DryLab model looks strange. What could be wrong?

Strange looking resolution maps and grayed-out or extra wide peaks are typically the result of errors in data entry.  

First, double-check that you’ve correctly entered your column dimensions and instrument data (such as dwell volume, etc.). Then, check that you’ve uploaded your *.cdf files in the correct order and that you’ve entered your data in the mode selection from lowest to highest.

If this does not solve your problem, please contact us for further technical support.  

9. I want to follow QbD guidelines during method development. Will DryLab help?

Yes! DryLab has been pioneering the development of analytical QbD in HPLC since 1986. Many of the present requirements such as Method Operable Design Region (MODR), the Edge of Failure, issues of Risk Assessment, Control Strategy, Continual Improvements, etc., can be easily studied in DryLab. The results are much higher flexibility in your HPLC methods, and faster commercial authorization. 

10. I upgraded to Windows 7 and now my old hardware key doesn't work. What do I do?

If you purchased DryLab after 2007, you will simply need to install the new driver for your hardware key. Please contact us for instructions. 

11. Is DryLab useful for teaching HPLC and for training new chromatographers?

Yes! Running a large number of experiments is time consuming and mistakes made at the instrument can be costly. DryLab lets beginners do their learning on the computer instead, and provides immediate feedback as they alter experimental conditions. DryLab also comeswith a tutorial guide and built-in examples for self-instruction.

We offer special teaching packages for universities – please contact us for more information.

12. Can DryLab be used for method transfer?

Yes! DryLab can predict chromatograms under various instrument and column conditions. You can adjust flow rate, dwell volume, extra column volume and column dimensions without needing to run any additional experiments.  

13. What are the hardware and software requirements to run DryLab?

System Software Requirements:

  • Windows 2000
  • Windows XP (Home or Professional)
  • Windows Vista
  • Windows 7
  • Windows 8

Hardware Requirements (Minimum):

  • Pentium III processor or comparable
  • 1024 MB of RAM
  • A color monitor, 1024x768 display with 16-bit color depth
  • 45 MB of free hard disk space (this includes room for storage of your DryLab data files)
  • The DryLab key that is included in your software package (if required) 

Hardware Requirements (Recommended):

  • 4096 MB of RAM
  • A color monitor, 1920x1080 display with 32-bit color depth
  • 90 MB of free hard disk space (this includes room for storage of your DryLab data files)
  • The DryLab key that is included in your software package (if required) 
14. Ok, I’m convinced! How do I purchase DryLab?

You can purchase DryLab directly through us at Molnár-Institute. Please contact us by phone (+49 30 421 5590) or by email to request a quotation and/or more information.