Literature

You are here:

Literature

So far, more than 260 peer reviewed papers have been published on the application of DryLab – a complete list of which you can find here.

DryLab draws on the philosophy described in the three most famous Solvophobic Theory papers I, II, III of Csaba Horváth, which were developed in the years 1975-1977 at Yale University (see also literature by Dr. Imre Molnár).

Read more about the Fundamentals of DryLab and its History.

Rapid Analysis of Peptide Mixtures by High Performance Liquid Chromatography With Nonpolar Stationary Phases

I. Molnár, Cs. Horváth
Peptides (Proc. 5th Am. Peptide Symp.), M. Goodman, J. Meienhofer, (Wiley, New York, 1977), 48-51

PDF

Charcoal, a nonpolar sorbent, had been widely used for the separation of peptides1 before the advent of ion-exchange chromatography. Recent developments in high performance liquid chromatography revived the interest in the use of nonpolar stationary phases for the separation of biological substances by “reversed phase” chromatography, which employs columns packed with 5 or 10µm porous silica particles having hydrocarbonaceous functions covalently bound to the surface.

This report illustrates the potential of this type of chromatography for the rapid analysis of minute quantities of peptide mixtures. The results suggest that octadecyl-silica columns can be used for fast separation of a wide variety of peptides. By monitoring the column effluent with a UV-detector at 200 nm, the sample components can be analyzed at the subnanomole level without the formation of UV absorbing or fluorescent derivatives.

Solvophobic Interactions in Liquid Chromatography with Nonpolar Stationary Phases – Solvophobic Interactions Part I

Csaba Horváth, Wayne Melander, Imre Molnár
Journal of Chromatography , 125, 129-156 (1976)

PDF
http://doi.org/10.1016/S0021-9673(00)93816-0

Solute interaction with nonpolar stationary phases in liquid chromatography is examined on the basis of the solvophobic theory. The chromatographic process is viewed as a reversible association of the solute with the hydrocarbonaceous ligands of bonded phases. A detailed analysis of the effect of the solvent on this process yields an expression for the capacity factor with essentially no adjustable constants. The theory satisfactorily accounts for the factors affecting solute retention under a wide range of experimental conditions. It makes possible the characterization of the solvophobic (eluent) strength of mixed solvents having different composition and the evaluation of the various solvophobic forces representing incremental values of the logarithm of the capacity factor. The wide applicability of nonpolar stationary phases (reversed phases) in liquid chromatography is demonstrated by the rapid separation of biogenic acids and bases on octadecylsilica columns with neat aqueous elements.

BACK 1 ...