DryLab® Analytical Design Space Modeling

Understand What Drives Your Separation—and Get the Most Out of It.

Turn a small set of experiments into a predictive model of your separation. Quantify how conditions affect retention and resolution, explore alternatives digitally, and identify robust operating ranges. Build the chromatographic understanding to solve demanding separation problems with fewer experiments.

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From a Few Experiments to a Million Predicted Separations.

Explore how conditions affect retention and resolution—digitally, with just one click.

Color-coded 3D resolution cube from DryLab®, showing how separation quality varies across method parameters

Over 99% Agreement Between Predicted and Measured Retention Times.¹

Know where your peaks will elute—before you run the experiment.

DryLab® predicted chromatogram stacked above the experimentally measured one: the peaks for impurities C, D, A, B, F, G and the API line up closely

3D Design Spaces Reveal What Works—and Where.

Identify conditions that meet all your defined separation criteria—at once.

3D design space: a wireframe cube whose red regions mark the parameter combinations that meet all separation criteria

Calculated Savings in Routine QC

Potential time and solvent savings based on published ebastine method data and a defined routine QC scenario.

97% saved on method runtime, 92% saved on development time

Design the Method That Meets Your Separation Criteria.

Adjust flow rate, column dimensions, and instrument parameters in your digital model. See your predicted chromatograms update instantly, showing how each change affects your separation.

Word cloud of DryLab® terms such as method development, robustness, design space, retention modeling and QbD, with DryLab® 4 at its center

Compare Separation Systems. Know Your Options.

Compare digital fingerprints to understand differences, identify suitable alternatives, and find conditions that meet your separation criteria.

Two HPLC columns, A and B, linked by glowing lines to a transparent cube that holds the 3D design spaces of both; the shared region marks shared operating conditions

Transform Your Experimental Data into Analytical Method Understanding.

Originally developed by Lloyd Snyder and fellow researchers, DryLab® builds on established chromatographic principles. It supports science-based, risk-informed method development—from selecting suitable working conditions to assessing robustness and planning verification experiments.

Using data from a small set of experiments, DryLab® builds a predictive model of your separation. Explore how method parameters affect retention and resolution, identify conditions that meet your separation criteria, and understand which parameters need tight control.

DryLab ↔ CDS Data Transfer

Create methods and sequences in Empower or OpenLab and retrieve processed results.

Peak Identification & Matching

Match peaks across input experiments, then prepare and review the peak table for your separation model.

3D Design Space Modeling

Explore how multiple method parameters interact and identify regions that meet your separation criteria.

Interactive Chromatogram Prediction

Navigate your design space and view predicted chromatograms as conditions change.

Gradient Editor

Adjust gradient steps in your model and immediately see their effect on your separation's retention and resolution.

Robustness Assessment

Evaluate how instrument tolerances and parameter variations affect your separation—and identify which variables need tight control to meet your performance criteria.

Knowledge Management & Documentation

Document method requirements, input data, model results, and your scientific rationale—to support lifecycle management and your method control strategy.

DryLab® software showing matched peak tracking and peak table generation

DryLab® software showing a multi-dimensional resolution model and working point selection

DryLab® software showing additional method parameter evaluation

DryLab® software showing robustness threshold testing across separation parameters

DryLab® software showing a generated GMP-compliant Knowledge Management report

All the must-haves for better separations

Stop guessing. Take control. Master your HPLC Method Development.

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Comparing DryLab® vs. other available packages

Feature	DryLab®	Other Softwares	What it means
Predictive Accuracy	>99% accuracy in predicting retention times and resolution from just a few runs.	Often relies on statistical or AI-based pattern recognition with variable accuracy.	Trust your model. DryLab® reveals what will happen, not just what might — minimizing lab work, wasted runs, and risk.
Scientific Foundation	Based on true chromatographic theory and physico-chemical modeling.	Primarily statistical, DOE, or AI-driven; often a “black box.”	Gain understanding, not just numbers — know why your separation behaves the way it does.
Experiment Efficiency	Requires only 2–12 input experiments to explore millions of possibilities.	Typically needs larger DOE matrices or automated run cycles.	Achieve deep insight with minimal bench work — save solvents, time, and resources.
Visualization & Design Space	Intuitive 3D models and Gradient Editor visualize resolution landscapes in real time.	Limited or basic 2D DOE plots.	See your chromatographic world in full color — understand complex interactions instantly.
Peak Tracking & Separation Control	Mechanistic peak tracking handles co-elution, shifting, and complex separations.	AI or algorithmic matching may miss subtle retention shifts.	Master even the most difficult separations — confidently separate what others can’t.
Regulatory & QbD Alignment	Built for ICH Q8, Q9, Q14, QbD, and GMP — produces fully compliant reports for submission.	Many tools support QbD but are often incomplete or require manual documentation.	Stay audit-ready with automated GMP reports that justify every parameter choice.
Robustness & Method Transfer	Models up to six parameters for robustness testing and transfer across instruments.	Often limited to screening or statistical robustness assessment.	Transfer methods seamlessly — no guesswork, no surprises in validation.
Knowledge Sharing & Reporting	Generates comprehensive Knowledge Management reports and clear documentation.	Often exports data; documentation requires manual effort.	Turn method development into organizational knowledge — capture, share, and justify every insight.
Scientific Credibility, Rigor and Validation	Supported by decades of peer-reviewed publications and regulatory adoption.	Few peer-reviewed validations; mostly vendor case studies.	Trust built on science. DryLab® stands on proven ground — used by leading pharma, CROs, and academia.
Human Support & Expertise	Backed by direct consulting, workshops, and decades of chromatographic expertise.	Primarily software support or sales-led guidance.	Learn from scientists, not sales reps — every interaction is a masterclass in method development.

I have been using DryLab since my PhD studies and continue to rely on it in my current role at Bayer AG. DryLab allows us to efficiently predict retention behavior and assess the impact of changing compound mixtures, significantly reducing experimental effort during method development. Its strong scientific foundation, based on established chromatographic models, delivers reliable results, while the software continues to evolve with valuable new capabilities. Beyond its practical benefits, DryLab is also an excellent learning tool, backed by a highly responsive and knowledgeable support team.

Ryan Karongo, PhD

Senior Scientist, Oligonucleotide and Peptide Analytics

Bayer AG

DryLab is an essential part of our HPLC method development process at Promega. Our analysts can quickly transition from their initial screening pass into DryLab, where the visualization of method parameters in 2D and 3D allows us to move the method into a robust and operable space. Before DryLab, we could not easily tell if a method was close to a failure point. By visualizing the model space, we can see these points clearly and adjust. The ability to simulate separation changes across a range of gradients, temperatures, and other parameters has decreased the time it takes us to develop a method. […]

DryLab is very helpful when we transfer methods from development to quality control. Design space visualization and robustness information provided by DryLab give us something we can share with our quality teams that we didn’t have before. This information gives our QA and QC teams confidence in the method.

The support from the DryLab team has been outstanding from the beginning. When we were evaluating different options, they worked closely with us, using data from our lab, to show all the features of the software and its benefits. Compared to other software we tried, DryLab is intuitive and easy to use for chromatographers with different levels of experience, and it stays focused on HPLC method development. After adoption, personalized, in-depth training sessions gave my team confidence to use the software right away. Since then, whenever we need help working through a model or have a problem, the DryLab team has been very responsive. They want to help us succeed. Their depth of experience and focus on customer support set them apart. We look forward to working with DryLab for many years to come.

Joshua Kimball

Senior Manager, Analytical Chemistry

Promega Corporation

I have used DryLab for many years across two different pharmaceutical companies. In my experience, DryLab has helped transform traditional trial-and-error method development into a Quality by Design approach aligned with current regulatory expectations. It provides valuable scientific insight, improves development efficiency, and has become an essential tool for robust UPLC method development.

Cristian Steiner

Analytical Development & Quality Testing Manager

Basilea

The use of hyphenated liquid chromatography-mass spectrometry (LC-MS) techniques has been supporting my research activity in the field of Analytical Chemistry. In most analytical challenges, I relied on high-resolution mass spectrometry (HRMS) as the primary separation domain, thus considering liquid chromatography as a simple support to HRMS-based detection. But what if your analytes are isomers with just slight structural differences? What if non-isomeric compounds share the same ionization channels due to in-source fragmentation phenomena? What if isomeric species exhibit minor differences in their complex fragmentation patterns? This is where HRMS faces its limits and the use of the chromatographic dimension becomes essential.

Whoever has a poor background in the field of chromatography and experimental design would rather choose a trial-and-error approach to improve the quality of the chromatographic separation. However, this can easily lead you to a negative spiral fed by waste of time, mental effort, and chemical resources. While I was facing the complexity of such an analytical scenario, I attended a talk by Dr. Imre Molnár focusing on DryLab. I came to their booth to get more information, and everything has changed since then.

[…] DryLab is your life jacket if you are drowning in the middle of desperate attempts to improve the quality of your chromatographic separation. With just a limited number of scouting experiments, the software allows you to optimize the quality of the separation with high accuracy in retention time prediction. Moreover, the use of the software improves the knowledge of your system, highlighting how the interaction between different factors (e.g., gradient steepness, column temperature, and the chemical composition of the mobile phase) can affect the quality of the separation. The use of DryLab also represented a learning opportunity in the field of chromatography due to a constant and fruitful scientific exchange with members from the Molnár Institute. Overall, DryLab is a must-have tool that boosted the quality and reliability of my scientific results. […]

It is often fascinating to observe how technological improvements in the field of separation science (e.g., ion mobility spectrometry, two-dimensional liquid and gas chromatography) can also push the research towards increasingly ambitious challenges. But it is also wise to ask yourself a simple question before trying to improve your lab instrumentation: did you really get the most out of your LC system? You should ask DryLab first.

Andrea Castellaneta, PhD

Postdoctoral Researcher

Università degli Studi di Bari Aldo Moro

We have relied on DryLab as the leading software for HPLC method development through simulations and modeling since our collaboration with Dr. Imre Molnar began in 1996. Over the years, DryLab has enabled us to develop robust, optimized gradient elution methods with remarkable efficiency. Its intuitive, user-friendly workflow empowers our team—especially those new to HPLC—to confidently design high-quality methods in just a few steps, eliminating the uncertainty often associated with conventional trial-and-error approaches. DryLab significantly reduces development time and solvent consumption, making HPLC method development faster, more cost-effective, and environmentally sustainable. We highly recommend DryLab to any laboratory engaged in HPLC gradient method development.

Professor Michael Lämmerhofer

Professor of Pharmaceutical (Bio)analysis

Universität Tübingen

As a Contract Research Organisation (CRO), Quality Assistance provides regulatory, scientific and technological expertise to large companies and SMEs in the pharmaceutical industry. Customers around the world rely on us when developing and marketing innovative human medicinal products in compliance with European and US regulations — also because we constantly invest in the most sophisticated technology and equipment. DryLab®'s Empower™ and OpenLab automated Design Space Modeling allows us to streamline the development of analytical procedures, both for small molecules and biotherapeutics. Knowing that our methods are optimised in a way that makes them robust for their subsequent use in any QC environment is a key reason why customers turn to us in product developments from discovery to the market place.

Arnaud Delobel, PhD

Director R&D

Quality Assistance S.A.

DryLab® optimizes the development of complex LC methods through an efficient, resource-saving approach. With only a few LC runs, robust methods can be reliably predicted, while robustness can be estimated without additional experiments. The software enables comprehensive method lifecycle management and allows flexible adjustment of LC method parameters within the design space without further experiments. The established design space is indispensable for a successful method transfer. Overall, DryLab® leads to significant savings in resources, time, equipment, and materials.

Ralf Hohl, PhD

Head of Laboratory

Sanofi

I've used DryLab for 10 years as an integral part of my approach to HPLC method development in pharmaceutical development, from early-stage drug candidates through late-stage programs. I have found it particularly valuable for challenging separations involving large, structurally complex small molecules with complicated impurity profiles, including methods where as many as 30 individual peaks needed to be separated. DryLab enables me to explore a much larger chromatographic design space than would be practical experimentally and, importantly, to better understand the factors driving selectivity between closely eluting components. For complex impurity profiles, this ability to model and visualize how changes in chromatographic conditions influence the entire separation can be extremely powerful. That capability is valuable throughout the stages of pharmaceutical development from rapidly identifying effective separation conditions for early-stage candidates to developing increasingly robust and well-understood methods as programs advance. DryLab helps me make more informed development decisions, reduce trial-and-error experimentation, and build greater confidence in method robustness. For me, DryLab is a powerful platform for understanding complex separations and supporting scientifically driven method development decisions.

Ashley Banks

Principal Scientist, Analytical Development

Kymera Therapeutics

DryLab has been central to my HPLC and UHPLC method development across research and industry for over 15 years. Having evaluated the platform's newest capabilities firsthand, I’ve found it consistently harmonizes scientific rigor, practical industry needs, and regulatory compliance. For analytical scientists seeking straightforward method development and deep chromatographic understanding with minimal investment of time and resources, DryLab is the absolute benchmark.

Róbert Kormány, PhD

Senior Structure Determination and Chromatography Expert

Egis Pharmaceuticals PLC

As a pharmaceutical service provider for AQbD-based method development and validation, Chromicent has successfully managed to keep ahead of the scientific and regulatory curve. One reason for this is our usage of DryLab® [...] it reduce screening and evaluation time drastically while ruling out any transfer errors by means of automation. More so, DryLab® helps us provide customers and regulators with information necessary to understand complex chromatographic behaviors. In addition, by using DryLab®, we fully comply with what ICH guidelines Q8, Q9, Q10, Q12 and Q14 expect.

Alexander Schmidt & Mijo Stanic

Founders

Chromicent

Based on a published ebastine verification study reporting a relative retention-time prediction error of 0.6%.Schmidt, A. H.; Molnár, I., Journal of Pharmaceutical and Biomedical Analysis 78–79 (2013), 65–74, Section 3.8 and Table 2. doi:10.1016/j.jpba.2013.01.032